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tRNA-derived SINEs are found in a number of eukaryotes especially mammalian genomes. After manual inspection and analysis, we apply the following filter to this genome for eliminating these tRNA-derived elements from true tRNAs. If the tRNA candidates have hits in both tRNAscan and Eufind first-pass scanners, we use a combination of at least 20 bits COVE score, 10 bits HMM score, and 5 bits 2' structure score to define a true tRNA. If the tRNA candidates only have hits in one of the first-pass scanners, the threshold of a true tRNA will be 55 bits COVE score.
| tRNAs Decoding Standard 20 AA | 430 | | Selenocysteine tRNAs (TCA) | 2 | | Possible suppressor tRNAs (CTA,TTA) | 1 | tRNAs with undetermined or unknown isotypes | 0 | | Predicted pseudogenes | 0 | | Total tRNAs | 433 |
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Intron Summary tRNAs with introns 24 | Ser AGA 1 | Arg TCT 4 | Leu CAA 4 | Ile TAT 4 | Met CAT 1 | Tyr GTA 10 |
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Four Box tRNA Sets | Isotype | tRNA Count by Anticodon | Total | | Ala | AGC 19 | GGC | CGC 10 | TGC 11 | 40 | | Gly | ACC 1 | GCC 14 | CCC 7 | TCC 7 | 29 | | Pro | AGG 7 | GGG | CGG 3 | TGG 8 | 18 | | Thr | AGT 9 | GGT | CGT 5 | TGT 4 | 18 | | Val | AAC 8 | GAC 1 | CAC 11 | TAC 3 | 23 |
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| Six Box tRNA Sets | Isotype | tRNA Count by Anticodon | Total | | Ser | AGA 9 | GGA 1 | CGA 3 | TGA 3 | ACT | GCT 8 |   |   | 24 | | Arg | ACG 6 | GCG | CCG 3 | TCG 5 |   |   | CCT 5 | TCT 5 | 24 | | Leu | AAG 8 | GAG | CAG 10 | TAG 3 |   |   | CAA 4 | TAA 4 | 29 |
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| Two Box tRNA Sets | Isotype | tRNA Count by Anticodon | Total | | Phe | AAA | GAA 7 |   |   | 7 | | Asn | ATT | GTT 14 |   |   | 14 | | Lys |   |   | CTT 19 | TTT 11 | 30 | | Asp | ATC | GTC 16 |   |   | 16 | | Glu |   |   | CTC 13 | TTC 8 | 21 | | His | ATG 1 | GTG 10 |   |   | 11 | | Gln |   |   | CTG 10 | TTG 6 | 16 |
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| Two Box & Other tRNA Sets | Isotype | tRNA Count by Anticodon | Total | | Ile | AAT 11 | GAT 1 |   | TAT 5 | 17 | | Met |   |   | CAT 18 |   | 18 | | Tyr | ATA | GTA 10 |   |   | 10 | | Supres |   |   | CTA | TTA 1 | 1 | | Cys | ACA | GCA 57 |   |   | 57 | | Trp |   |   | CCA 8 |   | 8 | | SelCys |   |   |   | TCA 2 | 2 |
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