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tRNA-derived SINEs are found in a number of eukaryotes especially mammalian genomes. After manual inspection and analysis, we apply the following filter to this genome for eliminating these tRNA-derived elements from true tRNAs. If the tRNA candidates have hits in both tRNAscan and Eufind first-pass scanners, we use a combination of at least 20 bits COVE score, 10 bits HMM score, and 5 bits 2' structure score to define a true tRNA. If the tRNA candidates only have hits in one of the first-pass scanners, the threshold of a true tRNA will be 55 bits COVE score.
| tRNAs Decoding Standard 20 AA | 437 | | Selenocysteine tRNAs (TCA) | 1 | | Possible suppressor tRNAs (CTA,TTA) | 0 | tRNAs with undetermined or unknown isotypes | 6 | | Predicted pseudogenes | 0 | | Total tRNAs | 444 |
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Intron Summary tRNAs with introns 23 | Thr AGT 1 | Arg TCT 6 | Leu CAA 3 | Phe AAA 1 | Asp ATC 1 | Ile TAT 3 | Tyr GTA 8 |
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Four Box tRNA Sets | Isotype | tRNA Count by Anticodon | Total | | Ala | AGC 34 | GGC 2 | CGC 4 | TGC 15 | 55 | | Gly | ACC | GCC 15 | CCC 5 | TCC 9 | 29 | | Pro | AGG 30 | GGG | CGG 3 | TGG 6 | 39 | | Thr | AGT 9 | GGT | CGT 3 | TGT 5 | 17 | | Val | AAC 8 | GAC | CAC 7 | TAC 5 | 20 |
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| Six Box tRNA Sets | Isotype | tRNA Count by Anticodon | Total | | Ser | AGA 8 | GGA | CGA 3 | TGA 4 | ACT 1 | GCT 9 |   |   | 25 | | Arg | ACG 6 | GCG | CCG 3 | TCG 5 |   |   | CCT 9 | TCT 8 | 31 | | Leu | AAG 6 | GAG | CAG 14 | TAG 4 |   |   | CAA 3 | TAA 2 | 29 |
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| Two Box tRNA Sets | Isotype | tRNA Count by Anticodon | Total | | Phe | AAA 1 | GAA 7 |   |   | 8 | | Asn | ATT | GTT 14 |   |   | 14 | | Lys |   |   | CTT 17 | TTT 9 | 26 | | Asp | ATC 1 | GTC 15 |   |   | 16 | | Glu |   |   | CTC 10 | TTC 10 | 20 | | His | ATG | GTG 12 |   |   | 12 | | Gln |   |   | CTG 9 | TTG 5 | 14 |
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| Two Box & Other tRNA Sets | Isotype | tRNA Count by Anticodon | Total | | Ile | AAT 12 | GAT |   | TAT 3 | 15 | | Met |   |   | CAT 13 |   | 13 | | Tyr | ATA | GTA 8 |   |   | 8 | | Supres |   |   | CTA | TTA | 0 | | Cys | ACA | GCA 38 |   |   | 38 | | Trp |   |   | CCA 8 |   | 8 | | SelCys |   |   |   | TCA 1 | 1 |
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